Adenovirus protein pVI binding to DNA and to major capsid protein
نویسندگان
چکیده
Background: The C-terminus of pVI activates the adenovirus proteinase. pVI escorts hexon into the nucleus. Results: pVI binds tightly to DNA independent of sequence, Kd = 46 nM. pVI binds tightly to hexon, Kd = 1.1 nM. Conclusion: DNA binding of pVI is first step in the activation of adenovirus proteinase. Significance: Links pVI, hexon, viral DNA and the adenovirus proteinase in virion maturation.
منابع مشابه
Regulation of a viral proteinase by a peptide and DNA in one-dimensional space: I. binding to DNA AND to hexon of the precursor to protein VI, pVI, of human adenovirus.
The precursor to adenovirus protein VI, pVI, is a multifunctional protein with different roles early and late in virus infection. Here, we focus on two roles late in infection, binding of pVI to DNA and to the major capsid protein hexon. pVI bound to DNA as a monomer independent of DNA sequence with an apparent equilibrium dissociation constant, K(d)((app)), of 46 nm. Bound to double-stranded D...
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